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KMID : 1041220050470010005
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Journal of Sericultural and Entomological Science
2005 Volume.47 No. 1 p.5 ~ p.11
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Efficient Inoculation Method of Beauveria bassiana for Production of Bombycis corpus and Evaluation of Its Liver Protection Activity
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Jung I-Yeon
Hong In-Pyo
Kang Pil-Don
Nam Sung-Hee
Kim Mi-Ja
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Abstract
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When inoculating with B. bassiana 101A for the mass production of B. corpus, the infection ratio was high with regardless of the treating time with highly-humidity if the concentration of spore was 1.0${\times}$$10^8$ spores/m/, but that was low if the concentration was 1.0${\times}$$10^7$ spores/m/. In the study of the activities according to the coserving temperature or days of the B. bassiana spawn, the infection ratio of 90% was maintained for 12 days in the temperature of $4^{\circ}C$. However, the infection ratio was rapidly dropped to the below of 5% after conserved for 48 hours in the temperature of $25^{\circ}C$. Besides, the activities of the original isolate had no difference after conserved for 12 months in the temperature of $4^{\circ}C$, so that the infection ratio 90% could be mintatined. In the measure of liver-protecting activities of B. bassiana 101A, the recovering effect was 43.5% and 65.7% respectively in the poisonous treatment induced with galactosamine, compared with liver-protecting activities of Silymarin and DDB in the $H_2O$ fraction. In the poisonous treatment induced with $CCl_4$ the recovering effect was 100% and 69.3% respectively, compared with liver-protecting activities of Silymarin and DDB in the EtOAc fraction.
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KEYWORD
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Bombyx, mori, Silymarin, B. bassiana
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